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| CITIZENSHIP | United States & Canadian |
| EDUCATION | Centenary College of Louisiana, Shreveport, LA
B.S. in Biology, awarded 1980 |
| Louisiana State University School of Graduate Studies, Shreveport, LA
Ph.D. in Cellular Biology and Anatomy, awarded 1990 (as Mary G. Oliver) |
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| PROFESSIONAL BACKGROUND | October 2001 - Present
Adjunct Professor American Intercontinental University, Los Angeles, CA |
| July, 1992-July, 1995
Post-doctoral Research Fellow, Dept. of Biochemistry Research Institute, Hospital for Sick Children, Toronto, Ontario |
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| January, 1991-July, 1992
Post-doctoral Research Fellow, Leukocyte Biology Laboratory Department of Pediatrics, Baylor College of Medicine, Houston, TX |
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| June 1990-January 1991
Post-doctoral Research Fellow, Dept. Cellular Biology and Anatomy L.S.U. School of Medicine, Shreveport, LA |
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| August 1985-June 1990
Graduate Research Assistant, Dept. Cellular Biology and Anatomy L.S.U. School of Medicine, Shreveport, LA |
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| August 1980-August 1985
Medical Research Technologist, Department of Anatomy L.S.U. School of Medicine, Shreveport, LA |
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| FELLOWSHIPS, HONORS and AWARDS | Research Fellowship, Cystic Fibrosis Foundation, 1993-1995
Who's Who in American Colleges and Universities Travel Award, American Association of Anatomists, 1986 |
| Travel Award, American Association of Anatomists, 1988 | |
| Travel Award, American Association of Anatomists, 1990 | |
| Travel Award, American Gastroenterological Association, 1990 | |
| Travel Award, FASEB Summer Conference, 1990 | |
| PUBLICATIONS | Invited Reviews |
| 1. Specian, Robert D. and Mary G. Oliver. Functional biology of the goblet cell. Am. J. Physiol. 260 (Cell Physiol. 29): C183-C193, 1991.
2. Forstner, Janet F., Mary G. Oliver, and Francisco A. Sylvester. Production, Structure, and Biologic Relevance of Gastrointestinal Mucins. In: Infections of the Gastrointestinal Tract, M.J. Blaser et al., editors. New York: Raven Press, Ltd., 1995. |
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| Journal Articles | |
| 1. Oliver, M.G., S.S. Wiggins and R.D. Specian. Tissue preparation of immunocytochemical localization of goblet cell mucin. Trans. Am. Microsc. Soc. 109: 205-212, 1990. | |
| 2. Specian, R.D. and M. G. Oliver. Rat intestinal mucin: an immunocytochemical study. Trans. Am. Microsc. Soc. 109: 213-222, 1990. | |
| 3. Oliver, M.G. and R.D. Specian. The cytoskeleton of intestinal goblet cells: the role of actin filaments in baseline secretion. Am. J. Physiol. 259 (Gastrointest. Liver Physiol. 22): G991-G997, 1990. | |
| 4. Radwan, K.A., M.G. Oliver and R.D. Specian. Cytoarchitectural reorganization of rabbit colonic goblet cells during baseline secretion. Am. J. Anat. 189: 365-376, 1990. | |
| 5. Weldon, R.A., J.W. Wills, C.R. Erdie, and M.G. Oliver. Incorporation of chimeric Gag protein into retroviral particles. J. Virology 64: 4169-4179, 1990. | |
| 6. Oliver, M.G. and R.D. Specian. The cytoskeleton of the intestinal goblet cell: the role of microtubules in baseline secretion. Am. J. Physiol. 260 (Gastrointest. Liver Physiol. 23): G850-G857, 1991. | |
| 7. Oliver, M.G. and R.D. Specian. Intracellular variation of rat intestinal mucin localized by monoclonal antibodies. Anat. Rec. 230:513-518, 1991. | |
| 8. Oliver, M.G., R.D. Specian, M.A. Perry and D.N. Granger. Morphologic assessment of leukocyte-endothelial cell interactions in mesenteric venules subjected to ischemia and reperfusion. Inflammation 15: 331-346, 1991. | |
| 9. Auchampach, J.A., M.G. Oliver, D.C. Anderson and A.M. Manning. Cloning, sequence comparison, and in vivo expression of the gene encoding rat P-selectin. Gene 145: 251-255, 1994. | |
| 10. Manning, A.M., H. Lu, G.L. Kukielka, M.G. Oliver, T. Ty, C.A. Toman, R.F. Drong, J.L. Slightom, C.M. Ballantine, M.L. Entman, C.W. Smith and D.C. Anderson. Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1). Gene 156: 291-295, 1995. | |
| 11. Kent, G., M.G. Oliver, J.K. Foskett M. Frdnova, P. Durie,J. Forstner, G. Forstner, J.R. Riordan, D. Percy and M. Buchwald. Phenotypic abnormalities in long term survival Cftr- mice. Pediatr. Res. 40: 233-241, 1996. | |
| 12. Rozmahel, R., M. Wilschanski, A. Matin, S. Plyte, M. Oliver, W. Auerbach, A. Moore, J. Forstner, P. Durie, J. Nadeau, C. Bear and L.C. Tsui. Modulation of disease severity in cystic fibrosis transmembrane conductance regulator deficient mice by a secondary genetic factor. Nat. Genetics 12: 280-287, 1996. | |
| Abstracts and Presentations | |
| 1. Specian, R.D. and M.G. Oliver. An immunofluorescent analysis of the biochemical methods for the quantitation of intestinal mucus secretion. Cystic Fibrosis Club (May, 1983). | |
| 2. Specian, R.D., C. M. Meyers, and M.G. Oliver. Morphometric analysis of intestinal goblet cells during maturation in rabbit colon. American Association of Anatomists (April, 1984). Anat. Rec. 208: 172a, 1984. | |
| 3. Wiggins, S.S. M.G. Oliver and R.D. Specian. An analysis of tissue fixation for immunocytochemistry in intestinal goblet cells. Student Research Forum, LSU School of Medicine in Shreveport (April, 1984). | |
| 4. Meyers, C.M., M.G. Oliver and R.D. Specian. Development of intestinal goblet cells in adult rabbit colon. Student Research Forum, LSU School of Medicine in Shreveport (April, 1984). | |
| 5. Oliver, M.G., J.W. Pickering and R.D. Specian. Monoclonal antibodies directed against rat intestinal mucus. American Association of Anatomists (May, 1985). Anat. Rec. 211: 143a, 1985. | |
| 6. Wiggins, S.S. M.G. Oliver and R.D. Specian. A quantitative analysis of the effects of cytoskeleton inhibitors on granule movement in intestinal goblet cells. American Association of Anatomists (May, 1985). Anat. Rec. 211: 214a, 1985. | |
| 7. Meyers, C.M., M.G. Oliver and R.D. Specian.. A morphometric analysis of goblet cells during accelerated secretion in rabbit colon. American Association of Anatomists (May, 1985). Anat. Rec. 211: 128a, 1985. | |
| 8. Oliver, M.G. and R.D. Specian. Rat intestinal mucins: an immunocytochemical study. American Association of Anatomists (April, 1986). Anat. Rec. 214: 93a-94a, 1986. | |
| 9. Oliver, M.G., R.D. Specian, R.E.F. Fahim and J.F. Forstner. Immunocytochemical localization of the link peptide in rat small intestinal mucins. Am. Soc. Cell Biol. (November, 1987). J. Cell Biol. 105: 160a, 1987. | |
| 10. Oliver, M.G. and R.D. Specian. Localization of IgA and secretory component in human intestinal goblet cells. American Association of Anatomists (April, 1988). Anat. Rec. 220: 72a, 1988. | |
| 11. Oliver, M.G., R.D. Specian, E. Deitch, J.F. Forstner, and D. McCool. Intragranular localization of secretory IgA in human intestinal goblet cells. American Society for Cell Biology (February, 1989). J. Cell Biol. 107: 351a, 1989. | |
| 12. Oliver, M.G., and R.D. Specian. The baseline secretory pathway in rabbit intestinal goblet cells. American Association of Anatomists (April, 1990). Anat. Rec. 226: 74a, 1990. | |
| 13. Specian, R.D., S.J. Zhang, D.A. Sibley, A.C. Kemper and M.G. Oliver. Recovery of goblet cells from an accelerated secretory event. American Association of Anatomists (April, 1990). Anat.Rec. 226: 97a, 1990. | |
| 14. Oliver, M.G., R.D. Specian, M.A. Perry and D.N. Granger. Endothelial permeability in response to ischemia/reperfusion. American Gastroenterological Association (May, 1990). Gastroenterology 98: A663, 1990. | |
| 15. Oliver, M.G. and R.D. Specian. Baseline secretion in rabbit colonic goblet cells. American Society for Cell Biology (Dec, 1990). J. Cell Biol. 111: 441a, 1990. | |
| 16. Specian, R.D., D.A. Sibley and M.G. Oliver. The intestinal mucus gel: a dynamic bactericidal and cytotoxic paradigm. American Association of Anatomists (April, 1991). Anat. Rec. 229: 85a, 1991. | |
| 17. Oliver, M.G., H. Jaeschke, J.L. Slightom, D.C. Anderson and A.M. Manning. Molecular cloning of rat P-selectin and studies of mRNA expression (November, 1992). Mol. Biol. Cell 3: 204a, 1992. | |
| 18. Rozmahel, R., S. Plyte, M. Oliver, M. Wilschanski, W. Auerbach, J. Forstner, C. Bear, A. Joyner, M. Buchwald and L.-C. Tsui. Characterization of mice homozygous for CFTR exon 1 disruption. North American CF Conference (October, 1994). Pediatric Pulmonology Supplement 10: 195, 1994. | |
| 19. Oliver, M.G., G. Kent and J.F. Forstner. Intestinal morphology of CFTR (-/-) mutant mice. American Society for Cell Biology (December 1994). Mol. Biol. Cell 5: 119a, 1994. | |
| TECHNICAL TRAINING | Morphological techniques |
| Light microscopy, including: tissue processing for cryotomy, paraffin, methacrylate and epoxy resins; thick and semi-thin microtomy; histological stains; immunocytochemistry, histochemistry; lectin cytochemistry; LM autoradiography; light micrography.
Electron microscopy, including: tissue processing for transmission EM; tissue processing (critical point drying, sputter coating) for scanning EM; untramicrotomy; preparation of support films; standard staining procedures; EM immunocytochemistry; electron micrography. | |
| Molecular Biology | |
| RNA analysis from mammalian sources, including: purification of total RNA and polyA RNA from whole tissue and cultured cells; Northern analysis; slot blot analysis.
cDNA production and analysis utilizing plasmid and phage vectors, including: cDNA library production and amplification; Southern analysis; subcloning; restriction mapping; dideoxy sequencing of double strand and single strand DNA templates; cycle-sequencing of single strand DNA templates; computer-assisted sequence analysis employing Eugene and SAM; polymerase chain reaction. | |
| Biochemistry | |
| Chromatography, including molecular sieve (gel filtration), ion exchange, and affinity chromatography.
Density gradient ultracentrifugation, fractionation, and purification of intestinal mucin glycoproteins. Polyacrylamide gel electrophoresis and Western blot methodology. Enzyme-linked immunosorbent assay (ELISA) methodology. Slot-blotting and histochemical analysis of glycoproteins and proteoglycans. | |
| Cell Culture Methodology | |
| Hybridoma technology, including: propagation of myeloma cell lines; spleen cell preparation; cell fusion methodology; hybridoma screening; propagation and cryopreservation of cell lines.
Epithelial cell line maintenance, propagation and preservation | |
| Darkroom Techniques | |
| Black and white film development, including 35mm and large format (4x5) film.
Black and white printing. Preparation of plates for publication, including preparation of copy negatives. | |
Copyright 1998-2002 Mary Gallien. All contents, unless otherwise noted, are the property of Mary Gallien or used with permission of the copyright owner. All text and photos herein may not be reproduced or distributed without the express written consent of Mary Gallien, her official representative, or the copyright owner.
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